According to the report, leaders and activists of the Hasina-led Awami League and its affiliated organisations armed with firearms and sticks created obstacles to traffic on the Dhaka-Chittagong highway to disrupt the student movement.
It alleged that Hasina, Quader and Asaduzzaman ordered the shooting and attack on protesting students and the public.
Milon, who was returning home from a local fish market at that time, was shot in the chest and collapsed on the road.
He was taken to Pro-Active Medical College and Hospital in the area, where he was declared dead, the report said.
This raises the number of cases filed against Hasina after her ouster to more than a dozen.
Over 230 people were killed in the incidents of violence that erupted across the country following the fall of the Hasina-led Awami League government, taking the death toll to more than 600 since the massive protest by students first started in mid-July.
An interim government was formed after the fall of the Hasina-led government, and 84-year-old Nobel laureate Muhammad Yunus was appointed as its Chief Adviser.